Programmable autonomous synthesis of single-stranded DNA

نویسندگان
چکیده

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Programmable autonomous synthesis of single-stranded DNA.

DNA performs diverse functional roles in biology, nanotechnology and biotechnology, but current methods for autonomously synthesizing arbitrary single-stranded DNA are limited. Here, we introduce the concept of primer exchange reaction (PER) cascades, which grow nascent single-stranded DNA with user-specified sequences following prescribed reaction pathways. PER synthesis happens in a programma...

متن کامل

Single-stranded DNA repeat synthesis by telomerase.

The eukaryotic ribonucleoprotein reverse transcriptase (RT) telomerase uses a template within its integral RNA subunit to extend chromosome ends by synthesis of single-stranded telomeric repeats. Telomerase is adapted to its unique cellular role by an ability to release product DNA in single-stranded form, regenerating free template from the product-template hybrid. Furthermore, by retaining a ...

متن کامل

Recyclable single-stranded DNA template for synthesis of siRNAs.

RNA interference is a post-transcriptional silencing mechanism triggered by the bioavailability and/or exogenous introduction of double-stranded RNA (dsRNA) into cells. Here we describe a novel method for the synthesis of siRNA in a single vessel. The method employs in vitro transcription and a single-stranded DNA (ssDNA) template and design, which incorporates upon self-annealing, two promoter...

متن کامل

Autonomous programmable DNA nanorobotic devices using DNAzymes

A major challenge in nanoscience is the design of synthetic molecular devices that run autonomously (that is, without externally mediated changes per work-cycle) and are programmable (that is, their behavior can be modified without complete redesign of the device). DNA-based synthetic molecular devices have the advantage of being relatively simple to design and engineer, due to the predictable ...

متن کامل

Single-stranded DNA-binding Proteins

The emergence of DNA as the predominant hereditary material of life has necessitated the evolution of proteins whose sole function is the maintenance and care of these vital molecules. In a typical cell, there is a wide array of proteins with specialized functions in DNA metabolism that allow the cell to maintain and replicate its genome. In order to copy or make repairs to DNA, the double heli...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Nature Chemistry

سال: 2017

ISSN: 1755-4330,1755-4349

DOI: 10.1038/nchem.2872